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Where do the images and cartoons in biology and medicine textbooks come from, and what will they look like in the future? How can we engineer and visualize, in 4D, cellular functions in development and disease? And how are these technologies being harnessed today to develop novel genetic and tissue engineering-based medical therapeutics? This course will convey the experience of the modern day experimental biologist. It will show how biomedical scientists culture organs and engineer stem cells and tissues outside the body, and how they visualize them with unprecedented spatiotemporal resolution (3D+Time=4D). The course will include an overview of different techniques in cell culture, genetic engineering, and imaging, followed by a live session using different light and electron microscopes, and finally, extended hands-on sessions on image analysis and quantification. The course will also include one or two guest lectures from premiere Hopkins researchers.