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This course will introduce students to high-throughput sequencing and its impact on biological research. At the end of the course students should be familiar with the various HTS techniques and how to use them for a project of their interest. They will also gain a clear understanding of the work flow involved and be familiar with commonly used bioinformatic tools.. The course will include both class room instruction and lab work (both wet lab and computer lab). The topics that will be covered include: High-throughput sequencing(HTS) technology - An introduction to the various technologies available, how they work, and the pros and cons for each of the current HTS technologies; A look at some of the most interesting/informative assays developed using HTS technology; Basics of command-line(unix) and the use of bioinformatic tools to analyze HTS data sets; An introduction to some well-established bioinformatic pipelines and; Critical evaluation and validation of results from HTS.