Advanced Research Lab in Cell and Molecular Biology: Single Molecule Imaging of the Epigenome
3.0
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An intensive research laboratory course on single-molecule imaging of epigenome proteins designed for undergraduate students with interests in biochemistry, biophysics, molecular, cellular and computational biology. The course introduces the use of advanced fluorescence microscopy to observe the single-molecule dynamics of key chromatin and transcription factors that control genome functions. Students will be instructed in one of two experimental approaches: 1) single-molecule imaging of fluorescently labeled chromatin protein factors in living cells; 2) single-molecule imaging of the interactions of protein factors with immobilized chromatin substrates in vitro. For both approaches, students will learn and apply imaging and computational tools to localize and track single protein molecules in real time and calculate their diffusive parameters. Students will interpret and integrate data to acquire conceptual insights on chromatin functions. Includes lectures, literature readings, student presentations. Two afternoons (8 hours lab and lectures/week, lab days flexible). Post-course research option in the Wu laboratory. Open to advanced sophomores or upper-level students with permission of Professor Carl Wu (wuc@jhu.edu) The semester course fulfills 3 credits towards the undergraduate B.S. research requirement for the Molecular and Cellular Biology (MCB) Program.
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