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This course examines how recently developed single-molecule methods have enhanced our understanding of cellular processes. The ability to observe and manipulate individual biological macromolecules has revolutionized our understanding of the machines and processes that enable life. The course will provide an overview of single-molecule approaches and discuss relevant publications that exemplify how these methodologies are applied to biological problems. For each approach, key concepts will be introduced in a lecture/discussion, followed by a student-led presentation of a related publication. Recommended coursework: Physics II