Semester.ly

Johns Hopkins University | AS.250.253

Protein Engineering and Biochemistry Lab

3.0

credits

Average Course Rating

(4.19)

This laboratory examines the relationship between genes and proteins in the context of disease and evolution. It is a research project lab in which the structural and functional consequences of mutations are determined for a model protein. Students will learn basic protein science and standard biochemical techniques and methods in protein engineering. They will perform experiments in site-directed mutagenesis, protein purification, and structural, functional and physical characterization of proteins. No prerequisites. Courses offered in Fall and Spring semesters.

Fall 2013

(3.94)

Fall 2014

(3.85)

Fall 2022

(3.94)

Spring 2013

(3.75)

Spring 2014

(4.63)

Spring 2015

(4.6)

Spring 2023

(4.21)

Spring 2023

(4.63)

Fall 2013

Professor: Carolyn Fitch

(3.94)

Students found that the best aspects of this course included the lab experiments, which they felt were very interesting and informative. Students real y enjoyed the hands-on aspects of this lab and appreciated the many skills they learned during the lab. However, students felt that many of the labs were disorganized and poorly explained, which often made them confusing and time consuming. Suggestions for improvement included breaking the course into two days, one for lecture, one for lab, so that the key concepts and processes for the lab could be ful y explained before students started a lab. Students also wanted more structure and requested more detailed information about what was expected of them for each lab and having explicit directions. Prospective students should be prepared for extensive amounts of work. They do not need much prior knowledge of the subject, but should be wil ing to participate and ask questions when they are confused or want additional clarification of concepts.

Fall 2014

Professor: Carolyn Fitch

(3.85)

Students praised this course for giving them the ability not only to learn new skil s but apply those skills as well. Issues with the course varied, though multiple students found there was a steep learning curve in the first few weeks and that feedback wasn’t provided in a timely manner to students resulting in repeating costly mistakes on assignments. Similarly, suggestions for improvement focused on a general request that assignments be better spaced out to al ow time for feedback in between the due date for the next assignment, or that feedback on assignments simply be returned to students more quickly. Prospective students should know that having some background in general lab procedure and programming is helpful for this course, although the class is accessible to a freshman.

Fall 2022

Professor: Jaime Sorenson

(3.94)

Spring 2013

Professor: Carolyn Fitch

(3.75)

The best aspects of the course included the interesting course material and the professor. The lectures were informative and well-organized. The worst aspects of the course included the assigned lab readings and the difficulty level of the course for students who did not have prior Biochemistry knowledge. The course would improve if there were more homework assignments and exams spread throughout the semester to give students a better understanding of the concepts. Prospective students should ensure they attend al classes and keep up with the lectures and labs.

Spring 2014

Professor: Carolyn Fitch

(4.63)

Most students agreed that the hands-on nature of the course and the student driven, individual investigations were the best features of this course. Learning various lab techniques and the experience of designing a mutation to a protein then carrying it out was unparalleled. The weekly homework was overwhelming at times, and some of the assignment instructions were unclear. Suggested improvements included breaking the class meeting time into two days per week and lowering the amount of weekly work. While the workload can be daunting, prospective students should know that this is a fun and interesting course.

Spring 2015

Professor: Carolyn Fitch

(4.6)

The best part about this class was the intriguing and interesting labs that allowed for hands-on learning of difficult material. Students agreed that the smal class size al owed for much individual attention and feedback from the professor, and that the methods introduced in this course would be useful in other subject areas. Some students found that the material was taxing, especially in the beginning of the semester, and that expectations were not always clear. This class could be improved by including more helpful feedback on assignments. Prospective students should know that background reading for labs is extremely helpful.

Spring 2023

Professor: Jaime Sorenson

(4.21)

Spring 2023

Professor: Aaron Robinson

(4.63)

Lecture Sections

(01)

No location info
J. Sorenson
13:30 - 17:30

(02)

No location info
J. Sorenson
13:30 - 17:30

(05)

No location info
A. RobinsonJ. Sorenson
13:30 - 17:30

(07)

No location info
A. RobinsonJ. Sorenson
18:00 - 22:00

(09)

No location info
A. RobinsonJ. Sorenson
18:00 - 22:00

(08)

No location info
A. RobinsonJ. Sorenson
18:00 - 22:00