Semester.ly

Johns Hopkins University | AS.250.383

Molecular Biophysics Laboratory

3.0

credits

Average Course Rating

(4.44)

An advanced inquiry based laboratory course covering experimental biophysical techniques to introduce fundamental physical principles governing the structure/function relationship of biological macromolecules. Students will investigate a “model protein”, staphylococcal nuclease, the “hydrogen atom” of biophysics. Using a vast library of variants, the effect of small changes in protein sequence will be explored. A variety of techniques will be used to probe the equilibrium thermodynamics and kinetic properties of this system; chromatography, spectroscopy (UV-Vis, fluorescence, circular dichroism, nuclear magnetic resonance), calorimetry, analytical centrifugation, X-ray crystallography, mass spectroscopy, and computational methods as needed for analysis. These methods coupled with perturbations to the molecular environment (ligands, co-solvents, and temperature) will help to elucidate protein function. Prerequisite: Introduction to Scientific Computing (250.205) or equivalent. Biophysical Chemistry (250.372 or 020.370) or equivalent. Course taught in Fall and Spring.

Fall 2012

(5.02)

Spring 2014

(4.0)

Spring 2015

(4.29)

Fall 2012

Professor: Karen Fleming

(5.02)

The best aspects of this course included the overall excitement of learning a new language and the very passionate professors. The class size wasn’t very large which made it easy for students to participate and interact with one another. The worst aspects of the course included the sometimes confusing lectures and the lack of structure. Many students felt like they were al on different levels in terms of their language skill and that the professors needed to be more aware of that. The class would be improved if the lessons were more structured with more oral and reading practice. Prospective students should know that this is an enjoyable class, but they wil real y have to spend time studying the language, especially if they have no previous background in Arabic.

Spring 2014

Professor: Carolyn Fitch

(4.0)

Most students cited the small class size and challenge of the course as the best aspects. Additionally, learning to use some new techniques and instruments was a highlight. However, the instructor’s poor time management and disorganization were the worst aspects of the course. More structure to each session and better time management were suggested improvements. Prospective students should know that the course can be fun and interesting but also frustrating.

Spring 2015

Professor: BIOPHYSICS

(4.29)

The best aspects of the course included the smal class size, the hands-on experience with biophysical techniques, and the application of concepts learned in prior classes. Students found the professor to be very helpful at answering both theoretical and practical questions, and appreciated that al students were working on related but different projects. Some students felt that both the class schedule and the assignments were disorganized, and that the workload was overwhelming towards the end of the year. This class could be improved by offering students more prompt feedback on papers, and sharing more data. Prospective students should know that this class was fun and chal enging. 44

Lecture Sections

(01)

No location info
A. Robinson
13:30 - 17:30

(02)

No location info
A. Robinson
13:30 - 17:30