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In-depth exploration of biophysical methods used to study structure, thermodynamic stability, and biomolecular interactions of macromolecules. Techniques include Circular Dichroism, Fluorescence Spectroscopy, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Surface Plasmon Resonance, Analytical Ultracentrifugation, and robotics. Through both lectures and hands-on labs, students will gain an understanding of the theoretical principles and practical applications of these methods, analyzing real data to interpret biomolecular phenomena.