4.0

credits

Average Course Rating

This laboratory course consists of integrated exercises to give students hands-on experience with various molecular techniques. This innovative hybrid course is intended for advanced learners with extensive molecular biology experience who want to use current and emerging techniques commonly employed in government and industry. This course will review fundamental molecular biology research principles and summarize the process of converting a research-based laboratory into a clinical-level laboratory. The onsite laboratory learning experiences will include, but not be limited to: PCR optimization, quantitative real-time PCR, and control of gene expression by DNA sequencing in the clinical setup. The essential concepts discussed include setting up a clinical lab, writing Standard Operating Protocols (SOPs) at the clinical level, and applying for a CLIA certificate. Students will also be introduced to microarray analysis and the utilization of bioinformatics pipelines. Prerequisites: 410.601 Biochemistry or equivalent; 410.602 Molecular Biology or equivalent; 410.603 Advanced Cell Biology or equivalent; 410.656 Recombinant DNA Laboratory; or consent of program committee. SCI