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Introduction to mechanobiology, an emerging multidisciplinary field that encompasses the study of mechanisms by which cells sense and respond to mechanical signals. Mechanical signals regulate a wide range of biological behaviors, including cell differentiation, migration, polarization, and etc. This course covers how these forces influence tissue development, adaptation, regeneration, and aging, with a focus on cardiovascular and pulmonary systems, cancer, and tissue engineering. We will discuss both theoretical and empirical foundations alongside engineering methodologies for the application, quantification, and elucidation of mechanobiological mechanisms. The implications for human health and the progression of disease will be highlighted.