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This intense course is for NTCR fellows with a background in the life sciences. This course covers the physics and chemistry relevant to the design, synthesis, and characterization of nanoparticles and patterned surfaces for biological applications. Topics include nanoparticle synthesis, functionalization, surface engineering, and applications in diagnostics and therapeutics. The properties of semiconductor quantum dots and magnetic nanoparticles are reviewed along with techniques for nanoparticle manipulation, particle tracking, and bio-microrheology. Patterning tools including soft lithography, optical lithography, e-beam lithography, and template lithography is discussed. Electron and scanning probe microscopy are reviewed. Examples applications in cancer is drawn directly from research in participating NTCR research labs.