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This lab course is a cornerstone of the training that all NTCR fellows acquire. The main objective of the lab course is to reveal the basics of biological systems to engineers in the physical sciences and of physical systems to biologists. This lab course takes place in new state-of-the-art facilities that have been equipped with funding from the HHMI and the NSF. Lab skills learned include: (i) physical and chemical tools to characterize and manipulate the properties of surfaces and nanoparticles; (ii) synthesis, ligand-functionalization, characterization, and targeted cell intake of multi-functional nanoparticles (nanowires and quantum dots); and (iii) atomic force microscopy and quantitative fluorescence microscopy for biological and materials applications. For fellows coming with a physics/engineering background, they learn basics of mammalian cell culture, molecular biology, cell transfection/transformation, and blotting techniques. The course captain is Denis Wirtz (Depts. Chemical and Biomolecular Engineering, Oncology, Pathology and Director of the previously NCI-funded NTCR program and PSOC center). Recommended Course Background: EN.670.620 or EN.670.400