Fundamentals of Fluorescence and Confocal Microscopy
1.0
creditsAverage Course Rating
Teach fundamental concepts of fluorescence and confocal microscopy so that students can pose appropriate question for meaningful research results. Emphasis on computer-based tools, including ImageJ, Imaris and CellProfiler, for image preparation and analysis with sessions in computer lab almost every week, complementing lectures. Two additional lab days required for operating a generic fluorescence microscope and a confocal microscope, respectively, learning to avoid common errors that preclude quantitative image interpretation and evaluating signal-to-noise effects of confocal settings.
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