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Johns Hopkins University | PH.260.613

Techniques in Molecular Biology

3.0

credits

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Supplements the scientific classroom experience of students in biological science graduate programs by providing hands-on experience with essential core molecular biology techniques. Involves intensive hands-on laboratory instruction along with the science behind the applications. Focuses on the following methodologies: polymerase chain reaction (PCR) DNA amplification, quantitative PCR, DNA and protein gel chromatography, Western blotting, transformation of bacteria, and expression and purification of heterologous proteins by bacteria. Gives special attention to technical aspects of scientific reproducibility and rigor.

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